Ghrelin在驯鹿器官内的表达及定位检测

doi:10.11843/j.issn.0366-6964.2016.12.022

摘要/Abstract

摘要：

为了探索Ghrelin在驯鹿体内的表达及定位，采用实时荧光定量PCR（Real-time PCR）和免疫组织化学技术对其进行检测。Real-time PCR结果显示Ghrelin在所检测的17种器官中均有转录，在皱胃内的转录量最高，其次是胰、十二指肠、睾丸和食管，且转录量显著高于其他器官（P＜0.05)；免疫组化结果揭示Ghrelin的免疫阳性细胞在食管和皱胃内主要分布于黏膜层、黏膜下层和肌层；在瘤胃、网胃、瓣胃的黏膜层、黏膜下层中也可见Ghrelin的免疫阳性细胞，但肌层中未见表达；在肠道主要位于十二指肠、空肠、回肠、结肠的黏膜层、黏膜下层和肌层，尤其在肠绒毛和黏膜下层分布较多；在肝、胰、甲状腺、垂体前叶、睾丸、肺、肾、脾器官内均有Ghrelin的免疫阳性细胞。Real-time PCR和免疫组化结果显示Ghrelin在所检测的器官内均有分布，且在食管和胃肠道内表达量及分布范围最为广泛，这表明Ghrelin对驯鹿的消化系统可能存在一定的调节作用。

Abstract:

The Real-time PCR and Immunohistochemistry were used to examine the expression and localization of Ghrelin in the reindeer organs. The results of Real-time PCR indicated that Ghrelin was expressed in 17 kinds of organs and the expression in the abomasum was highest, followed by pancreas, duodenal, testis and oesophagus, in which the expression of Ghrelin were remarkably higher than that of the rest organs (P<0.05). Immunohistochemical results showed that Ghrelin-positive cells in the oesophagus and abomasum mainly distributed in the tunica mucosa, tunica submucosa and tunica muscularis and also detected in the tunica mucosa, tunica submucosa of rumen, reticulum, omasum. In the intestine, Ghrelin-positive cells were mainly localized in the tunica mucosa, tunica submucosa and tunica muscularis of duodenum, jejumum, ileum, colon, especially more cells were found in the epithelium of intestinal villi and the tunica submucosa. Ghrelin-positive cells were observed in the liver, pancreas, lung, spleen, kidney, testis, anterior pituitary, thyroid gland. Real-time PCR and immunohistochemistry results showed that Ghrelin was presented in all of the organs that were examined, where the expression and distribution of Ghrelin in the esophagus and gastrointestinal were most widely, suggesting its possible role in regulating the digestive function.

中图分类号:

S852.1

张曼，金鑫，刘骄，杨银凤. Ghrelin在驯鹿器官内的表达及定位检测[J]. 畜牧兽医学报, 2016, 47(12): 2510-2519.

ZHANG Man, JIN Xin, LIU Jiao, YANG Yin-feng. The Examination of Expression and Localization of Ghrelin in the Reindeer Organs[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2510-2519.

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